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Figure 5 | Plant Methods

Figure 5

From: Simple and efficient methods to generate split roots and grafted plants useful for long-distance signaling studies in Medicago truncatula and other small plants

Figure 5

Nodule number on inverted-Y grafted plants. (A) Wild type (A17) self grafts show systemic inhibition of nodulation on the second root. The X axis label indicates whether the first root inoculated (Root A) was the stock root or the inserted root. Root B was inoculated 4 days after Root A and data was collected 21 days after Root B inoculation. The Y axis is average nodule number per root; error bars indicate standard error of the mean. Both comparisons are significant by pair-wise t-test with P < 0.05. N = 6 for the first set of bars and 8 for the second. (B) Longitudinal section of vasculature at Y graft junction (C) Reciprocal graft longitudinal section. (D &E) Cross sections of stock root and grafted root respectively of plant in (B).

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Plant Methods

ISSN: 1746-4811