Figure 3

Test of interaction between sense and antisense OeSLG transcripts in anthers. The full-length long and short forms were cloned, amplified, precipitated, denatured, annealed and separated on an agarose gel in three combinations: sense/sense, sense/antisense and antisense/antisense. The sense/sense transcripts show a single band of 1,269 bp, the antisense/antisense transcripts show a single band of 780 bp, and the sense/antisense transcripts show three different bands, one higher, one lower and one of intermediate size, indicating that interactions between the sense and the antisense transcripts are possible.