Rapid quantification of plant-powdery mildew interactions by qPCR and conidiospore counts

Table 1 Primer sequences and amplification efficiencies

Primer	Sequence	Target	Accession#	Amplicon in bp	Efficiency in%
R189	GAATCCACCCATACCACCAG	RNA-binding (RRM/RBD/RNP motifs) family protein	At3g21215	114	95
R192	GAGGAGGAGGATGGTGATGA	RNA-binding (RRM/RBD/RNP motifs) family protein	At3g21215	114	95
R243	AAGCACCTCCTGCTGTTCAT	Glyceraldehyde-3-phosphate dehydrogenase of plastid 2	At1g16300	125	91
R244	CTTTCCACTGCTCCTTGACC	Glyceraldehyde-3-phosphate dehydrogenase of plastid 2	At1g16300	125	91
R193	TCGCCGCTATATTTGGAGTC	Plasma membrane ATPase 1	Go_V1_Contig3757	90	90
R194	CTGGGTCAGATGGTTCACCT	Plasma membrane ATPase 1	Go_V1_Contig3757	90	90
R263	TCTTGGTGGCACGAATGAC	GDSL-like lipase	Go_V1_Contig76	92	100
R264	AGTGCGAGAGTGGGACAGAC	GDSL-like lipase	Go_V1_Contig76	92	100

ISSN: 1746-4811