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Figure 1 | Plant Methods

Figure 1

From: Rapid quantification of plant-powdery mildew interactions by qPCR and conidiospore counts

Figure 1

Powdery mildew disease progression on Arabidopsis seedlings. (A) Schematic overview and microscopic images of powdery mildew disease progression on Col-0 seedlings. Samples were harvested at indicated time points and stained with Coomassie Brilliant Blue. Arrows indicate conidiospore chains and arrowheads point to the initial spore. hpi, hours post inoculation (B) qPCR analysis of a time series of powdery mildew infection on Col-0 wild type, eds1, mlo2 and mlo2 mlo6 mlo2 seedlings. Ratios of G. orontii to Arabidopsis gDNA were determined by qPCR with primers R189/R192 and R193/R194, respectively. Bars represent the mean ± standard deviation of three technical replicates from a DNA sample of ten pooled seedlings grown in five different pots (two seedlings/pot used). (C) qPCR analysis of powdery mildew infection on Arabidopsis mutants that show powdery mildew-induced cell death. Representative time points of infection on Col-0 wild type, eds1, mlo2, pmr4 and edr1 seedlings were used. Ratios of G. orontii to Arabidopsis gDNA were determined by qPCR with primers R189/R192 and R193/R194, respectively. Bars represent the mean ± standard deviation of three DNA samples (each derived from ten pooled seedlings grown in five different pots) with three technical replicates each. Asterisks indicate statistically significant differences to Col-0 in two-tailed Student’s t-test (p <0,05). Schematic overview in (A) is courtesy of Justine Lorek. Scale bars in (A) are 100 μm. Data shown are representative of three independent experiments (see Additional file2 for data of a second experiment).

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