Table 5 20 primers pairs designed according to SNPs between rapeseed zy036 and 51070
From: An improved allele-specific PCR primer design method for SNP marker analysis and its application
Primer name | SNP type | Mismatch site closest to 3’end of primers | Mismatch base pairs | Forward primer(5’-3’) | Reverse primer(5’-3’) |
|---|---|---|---|---|---|
Br94494 | C/G | 2nd | CT | AGTTACATAGGTCCACAATCATAGAATAAACTTTTC | TACCATTCGGAGCCTAAATAGAGGTAAAAGGTG |
Br27005 | T/A | 3rd | CA | AAGATTGTTTCAAACGCAAAAATATACAACAAAAT | CAACGAATTTCACACTTTAGTAATGCACTGAGATTT |
Br31054 | T/A | 3rd | CA | CAAGTGAGACTGAATCCACAATAAAGGATGCTACT | TCGGATAAAATCCCCAGCTCTACTATACATTCC |
Br43193 | T/A | 3rd | CA | CCTTTTATTTGATCACAGGGGTTTGTAGGAACT | TCAGCCAGTAACGTCCCCCACATC |
Br51190 | T/A | 3rd | CA | AAATAATGGCATGCTCCTCTTTAATCTACCAAACT | TATTCGGTTCCGAAAATAATGCGATGC |
Br34590 | T/A | 3rd | CA | AAGTGACGGTTCTTTAAGTTATCAGAGTCTCCTAAT | AGATTTGGGATTAAAATCAAGTTGTGGGTTAGTTTT |
Br03637 | T/A | 3rd | CA | ATTACAGAATGTGTGTGCAAACAGAAATACATTACT | TGTGTCCCCATTTCGTGTAATCATAAAGCTAG |
Br39807 | T/A | 3rd | CA | CGAGACTCGGGTCGTTGAGTGGAAAT | ATCCTAAAGACTTCTCCCACAAATCCACCAT |
Br68275 | T/A | 3rd | CA | TGCCGCATGTATGTCGGAGATGATAAT | AACCGAAACCCTAGTAGGCTAGGCGC |
Br29253 | T/A | 3rd | CA | TGGCGCTAAATCCAAGAAGAAGTCCATT | AATTACCACCTTTCTTACCCTTGTTACTCATGACAG |
Br61715 | T/A | 3rd | CA | TGAATAGATTCTTCCGCATCACCTTTTAAAGTTAAT | CTTGTTTCAAGAGAAATTGAACAAGCTGCAGT |
Br24494 | C/G | 2nd | GT | GTCAATAATACTAGCAAACATACAACAGCGAGATTC | TTGCAAATTTTAGTCAAAGTCGGTAGAAAATAGATC |
Br06710 | T/A | 3rd | CA | TCTTGTCGATGCTGAGCTGGCAAATACT | GGTCAAGCTCACACACACTCCACGTC |
Br00855 | T/A | 3rd | CA | CACTATGGGCTATGGTGGGTCCTTCAAT | TGATTGGAGTTCTGTGCTCGTAGTTTTGC |
Br71197 | C/G | 2nd | GT | TATGGCACACAGACAGAGTTCCAGGAAATC | TCTTCCAGTTCGATATCTTGGTCTGTCCC |
Br07809 | C/G | 2nd | GT | CTCCGCCCACATGTTATAATATGTCAGTATATCTTC | TAGTGAATGGAGAAAGAGAACAAAGCCTACAGTACA |
Br02456 | T/A | 3rd | CA | GCCTTCAGAAGGTCTGGAAACTGGATT | GTTCGATGGACTTCACTACCTCCCATAGCT |
Br77080 | T/A | 3rd | CA | CGGATAGTTTCGGGTTCGGTTCGATT | ACCGAACGGGTACCCGAATATATAAAAATATTAATT |
Br77646 | T/A | 3rd | CA | TCCACCAGAATTGTGTGATGGCACTTACT | GAAAAACGTCAGGTCAATGTATCAACTTCGATAA |
Br14184 | G/A | 3rd | AC | GATTAACCGATGAAAGTCTCAGTGCCACAG | TCGTCTGTGACTCCCAAACACTTGGATAG |