Figure 3
From: Universal Multiplex PCR: a novel method of simultaneous amplification of multiple DNA fragments
Comparison of UM-PCR products amplified using SSR primers and different types of adapter-primers. (A) Comparison of single PCR products and quintuple PCR products amplified using U-primers. M, 20 bp DNA Marker (Takara); lanes 1–4, U-phi085; lanes 5–8, U-phi041; from 9 to 12, U-phi123; lanes 13–16, U-umc1478; lanes 17–20, U-umc1268; lanes 21–24 contain five pairs of U-primers (U-phi085, U-phi041, U-phi123, U-umc1478 and U-umc1268). Zhengdan 958: lanes 1, 2, 5, 6, 9, 10, 13, 14, 17, 18, 21, 22; Xianyu 335: lanes 3, 4, 7, 8, 11, 12, 15, 16, 19, 20, 23, 24. (B) Comparison of quintuple PCR products amplified using SSR primers and U-primers. M, 20 bp DNA Marker (Takara); lanes 1–4 contain five pairs of SSR primers (phi085, phi041, phi123, umc1478 and umc1268); lanes 5–8 contain five pairs of U-primers (U-phi085, U-phi041, U-phi123, U-umc1478 and U-umc1268); Zhengdan 958: lanes 1, 2, 5, 6; Xianyu 335: lanes 3, 4, 7, 8. (C) Quintuple PCR products amplified using C-primers. Lanes 1–4 contain five pairs of C-primers (C-phi085, C-phi041, C-phi123, C-umc1478 and C-umc1268); M, 20 bp DNA Marker (Takara); Zhengdan 958: lanes 1, 2; Xianyu 335: lanes 3, 4. (D) Quintuple PCR products amplified using U-primers. Lanes 1–4 contain five pairs of U-primers (U-phi085, U-phi041, U-phi123, U-umc1268 and U-phi120); M, 20 bp DNA Marker (Takara); Zhengdan 958: lanes 1, 2; Xianyu 335: lanes 3, 4. (E) Quintuple PCR products amplified using U-primers. Lanes 1–4 contain five pairs of U-primers (U-phi041, U-phi123, U-umc1478, U-umc1268 and U-phi120); M, 20 bp DNA Marker (Takara); Zhengdan 958: lanes 1, 2; Xianyu 335: lanes 3, 4.