Figure 3
From: A simple and efficient transient transformation for hybrid aspen (Populus tremula × P. tremuloides)
Subcellular localization of Populus proteins and protein-protein interaction in hybrid aspen cells. (A-D) Transient GFP fusion protein expression assay in hybrid aspen leaves. (A) PttMTP1-GFP labels the tonoplast, (B) PttPrxQ-GFP labels the plastids, (C) PttC4H-GFP labels the ER and (D) PttGT47C-GFP labels the Golgi. After three days of the transformation, GFP signals, chlorophyll autofluorescence and fluorescence of organelle markers were captured using a CLSM. Columns show GFP fluorescence (left), fluorescence of organelle markers and chloroplast autofluorescence (middle, left), the superimposed images (Merged; middle, right), and bright field images (BF, right). Scale bar = 10 μm. (E) BiFC assay in aspen leaves using transient co-transformation. The plasmid constructs containing CaMV35S::nEYFP-AtRBR1 and CaMV35S::cEYFP-AtCYCD3;1 were used for BiFC assay. YFP signals and DAPI fluorescence were captured using a CLSM. Columns show YFP fluorescence (left), DAPI (middle, left), the superimposed images (Merged; middle, right), and bright field images (BF, right). Scale bar = 10 μm.