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Figure 5 | Plant Methods

Figure 5

From: Screening for in planta protein-protein interactions combining bimolecular fluorescence complementation with flow cytometry

Figure 5

Cytometric plots of testing for BIFC between bait CPK3-YN173 and fished YC fusion proteins. A. Cytometric plots are shown from the second round screening that lead to 8 positive colonies (3 were eliminated due to technical difficulties, see text). B. Subsequent testing of the 8 candidate proteins after re-cloning of the candidate cDNAs and tested against CPK3-YN173 or YN154-CPK3. C. The YFP BiFC index was calculated as previously published [12]; it is the mean times fluorescence intensity. These experiments were repeated in their entirety 3 times with 3 replicates per experiment with similar results. Colony #2 was delayed in cloning and therefore showed with its respective negative free-SPYCE control. n.d. = not done.

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Plant Methods

ISSN: 1746-4811