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Figure 4 | Plant Methods

Figure 4

From: Screening for in planta protein-protein interactions combining bimolecular fluorescence complementation with flow cytometry

Figure 4

Cytometric and plasmid recovery results from the CPK3 screen. Panels A- D show typical results of observing 2 x 105 to 4 x 105 events; more than 2.5 x 106 events were analyzed per FACS session. A. Mock transfected (water only), B. CPK3-YN173 transfected alone (no YFP signal is produced), C. First Round, initial co-transfection of CPK3-YN173 and YC-cDNA library, D. Second Round, co-transfection of the fished library plasmids of the first round, pooled challenged with bait CPK3-YN173, E. Number of positive BIFC cells sorted, the number of recovered bacterial colonies and the number of colonies producing positive BIFC in one-on-one challenges with the bait (actual plots are showed in Figure5).

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Plant Methods

ISSN: 1746-4811