A new approach for cytokinin isolation from Arabidopsis tissues using miniaturized purification: pipette tip solid-phase extraction

Table 4 Recovery (%) of different CK groups in relation to number of sorbent multi-layers (C18, SDB-RPS, Cation-SR) used in PT-SPE purification procedure

	Recovery (%)^a
CKs	Number of sorbent multi-layers a
	1	2	3
Free Bases	23 ± 3	58 ± 13	73 ± 15
Ribosides	11 ± 4	33 ± 9	77 ± 18
N- glucosides	7 ± 1	36 ± 23	85 ± 5
O- glucosides	7 ± 3	31 ± 21	81 ± 10
Nucleotides	5 ± 3	13 ± 2	81 ± 6

a Values are means ± SD (n = 4).
Plant tissues (5 mg FW of A. thaliana seedlings spiked with 1 pmol of authentic CK standards) were extracted in Bieleski buffer, purified by StageTip protocol and directly analyzed by UHPLC-ESI(+)-MS/MS.

ISSN: 1746-4811