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Table 4 Recovery (%) of different CK groups in relation to number of sorbent multi-layers (C18, SDB-RPS, Cation-SR) used in PT-SPE purification procedure

From: A new approach for cytokinin isolation from Arabidopsis tissues using miniaturized purification: pipette tip solid-phase extraction

  Recovery (%)a
CKs Number of sorbent multi-layers a
  1 2 3
Free Bases 23 ± 3 58 ± 13 73 ± 15
Ribosides 11 ± 4 33 ± 9 77 ± 18
N- glucosides 7 ± 1 36 ± 23 85 ± 5
O- glucosides 7 ± 3 31 ± 21 81 ± 10
Nucleotides 5 ± 3 13 ± 2 81 ± 6
  1. a Values are means ± SD (n = 4).
  2. Plant tissues (5 mg FW of A. thaliana seedlings spiked with 1 pmol of authentic CK standards) were extracted in Bieleski buffer, purified by StageTip protocol and directly analyzed by UHPLC-ESI(+)-MS/MS.