Table 3 Method validation
CKs | Recovery (%)a | Determined spiked CK content (pmol)b | Method precision (% RSD)b | Method accuracy (% bias)b | ||
|---|---|---|---|---|---|---|
1 mg FW | 2 mg FW | 5 mg FW | ||||
t Z | 80 ± 10 | 63 ± 8 | 21 ± 4 | 0.99 ± 0.17 | 17.2 | 0.1 |
t ZR | 72 ± 12 | 46 ± 7 | 8 ± 1 | 0.84 ± 0.09 | 10.4 | 16.1 |
t Z7G | 88 ± 6 | 57 ± 4 | 6 ± 1 | 0.91 ± 0.13 | 14.6 | 8.6 |
t Z9G | 59 ± 7 | 31 ± 3 | 8 ± 1 | 0.97 ± 0.09 | 9.6 | 2.5 |
t ZOG | 85 ± 5 | 68 ± 6 | 11 ± 2 | 1.07 ± 0.19 | 18.1 | −7.1 |
t ZROG | 55 ± 4 | 30 ± 3 | 4 ± 1 | 0.82 ± 0.09 | 11.1 | 17.6 |
t ZMP | 35 ± 6 | 11 ± 1 | 5 ± 1 | 0.85 ± 0.11 | 13.3 | 14.7 |
c Z | 75 ± 9 | 65 ± 5 | 24 ± 4 | 0.83 ± 0.02 | 2.5 | 17.0 |
c ZR | 81 ± 13 | 44 ± 9 | 8 ± 1 | 0.96 ± 0.12 | 12.8 | 4.1 |
c Z9G | 74 ± 12 | 37 ± 5 | 5 ± 1 | 1.18 ± 0.13 | 11.3 | −17.6 |
c ZOG | 89 ± 6 | 66 ± 7 | 9 ± 2 | 1.09 ± 0.14 | 13.2 | −9.1 |
c ZROG | 52 ± 6 | 24 ± 2 | 3 ± 1 | 0.89 ± 0.11 | 12.9 | 11.2 |
c ZMP | 32 ± 3 | 17 ± 1 | 2 ± 1 | 0.86 ± 0.15 | 17.9 | 13.9 |
DHZ | 77 ± 13 | 61 ± 7 | 20 ± 3 | 0.90 ± 0.10 | 10.8 | 9.7 |
DHZR | 88 ± 13 | 48 ± 8 | 12 ± 1 | 1.03 ± 0.06 | 5.6 | −2.9 |
DHZ7G | 89 ± 3 | 65 ± 3 | 8 ± 2 | 1.18 ± 0.05 | 4.3 | −18.2 |
DHZ9G | 78 ± 10 | 35 ± 6 | 6 ± 1 | 0.96 ± 0.07 | 7.2 | 3.7 |
DHZOG | 77 ± 5 | 50 ± 7 | 9 ± 3 | 1.16 ± 0.06 | 4.9 | −15.8 |
DHZROG | 87 ± 8 | 42 ± 5 | 5 ± 1 | 0.90 ± 0.12 | 13.3 | 9.8 |
DHZMP | 37 ± 1 | 12 ± 1 | 3 ± 1 | 0.96 ± 0.15 | 15.8 | 3.6 |
iP | 76 ± 9 | 68 ± 3 | 26 ± 5 | 0.97 ± 0.06 | 5.9 | 3.3 |
iPR | 84 ± 8 | 53 ± 4 | 17 ± 1 | 1.13 ± 0.06 | 5.6 | −12.8 |
iP7G | 83 ± 10 | 60 ± 5 | 7 ± 1 | 0.91 ± 0.15 | 16.5 | 9.4 |
iP9G | 74 ± 8 | 49 ± 8 | 8 ± 2 | 0.97 ± 0.08 | 8.0 | 3.1 |
iPMP | 78 ± 9 | 39 ± 9 | 9 ± 2 | 0.92 ± 0.16 | 17.0 | 8.4 |