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Table 3 Method validation

From: A new approach for cytokinin isolation from Arabidopsis tissues using miniaturized purification: pipette tip solid-phase extraction

CKs Recovery (%)a Determined spiked CK content (pmol)b Method precision (% RSD)b Method accuracy (% bias)b
  1 mg FW 2 mg FW 5 mg FW    
t Z 80 ± 10 63 ± 8 21 ± 4 0.99 ± 0.17 17.2 0.1
t ZR 72 ± 12 46 ± 7 8 ± 1 0.84 ± 0.09 10.4 16.1
t Z7G 88 ± 6 57 ± 4 6 ± 1 0.91 ± 0.13 14.6 8.6
t Z9G 59 ± 7 31 ± 3 8 ± 1 0.97 ± 0.09 9.6 2.5
t ZOG 85 ± 5 68 ± 6 11 ± 2 1.07 ± 0.19 18.1 −7.1
t ZROG 55 ± 4 30 ± 3 4 ± 1 0.82 ± 0.09 11.1 17.6
t ZMP 35 ± 6 11 ± 1 5 ± 1 0.85 ± 0.11 13.3 14.7
c Z 75 ± 9 65 ± 5 24 ± 4 0.83 ± 0.02 2.5 17.0
c ZR 81 ± 13 44 ± 9 8 ± 1 0.96 ± 0.12 12.8 4.1
c Z9G 74 ± 12 37 ± 5 5 ± 1 1.18 ± 0.13 11.3 −17.6
c ZOG 89 ± 6 66 ± 7 9 ± 2 1.09 ± 0.14 13.2 −9.1
c ZROG 52 ± 6 24 ± 2 3 ± 1 0.89 ± 0.11 12.9 11.2
c ZMP 32 ± 3 17 ± 1 2 ± 1 0.86 ± 0.15 17.9 13.9
DHZ 77 ± 13 61 ± 7 20 ± 3 0.90 ± 0.10 10.8 9.7
DHZR 88 ± 13 48 ± 8 12 ± 1 1.03 ± 0.06 5.6 −2.9
DHZ7G 89 ± 3 65 ± 3 8 ± 2 1.18 ± 0.05 4.3 −18.2
DHZ9G 78 ± 10 35 ± 6 6 ± 1 0.96 ± 0.07 7.2 3.7
DHZOG 77 ± 5 50 ± 7 9 ± 3 1.16 ± 0.06 4.9 −15.8
DHZROG 87 ± 8 42 ± 5 5 ± 1 0.90 ± 0.12 13.3 9.8
DHZMP 37 ± 1 12 ± 1 3 ± 1 0.96 ± 0.15 15.8 3.6
iP 76 ± 9 68 ± 3 26 ± 5 0.97 ± 0.06 5.9 3.3
iPR 84 ± 8 53 ± 4 17 ± 1 1.13 ± 0.06 5.6 −12.8
iP7G 83 ± 10 60 ± 5 7 ± 1 0.91 ± 0.15 16.5 9.4
iP9G 74 ± 8 49 ± 8 8 ± 2 0.97 ± 0.08 8.0 3.1
iPMP 78 ± 9 39 ± 9 9 ± 2 0.92 ± 0.16 17.0 8.4
  1. a Values are means ± SD (n = 4); b Values are means ± SD (n = 12).
  2. Recovery (%) is shown for different amounts of plant matrix together with determinations of the analytical precision and accuracy of whole procedure. Plant tissues (1–5 mg FW of A. thaliana seedlings spiked with 1 pmol of authentic CK standards) were extracted in Bieleski buffer, purified by multi-StageTip microcolumn chromatography and directly analyzed by UHPLC-ESI(+)-MS/MS.