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Table 2 Method parameters

From: A new approach for cytokinin isolation from Arabidopsis tissues using miniaturized purification: pipette tip solid-phase extraction

Compounds

Precursor

Products

Retention timea (min)

LODb (fmol)

Dynamic range (mol)

R 2

t/cZ

220.1

136.1, 119.0

15.39 ± 0.04/16.82 ± 0.03

0.5

1×10−15–5×10−11

0.9989/0.9987

t/cZR

352.2

220.1, 136.1

19.06 ± 0.02/19.72 ± 0.01

0.1

5×10−16–5×10−11

0.9993/0.9986

tZ7G

382.2

220.1, 136.1

12.26 ± 0.02

0.1

5×10−16–1×10−11

0.9989

t/cZ9G

14.23 ± 0.02/15.13 ± 0.02

0.1

5×10−16–5×10−11

0.9993/0.9985

t/cZOG

14.83 ± 0.03/15.79 ± 0.03

0.5

1×10−16–1×10−11

0.9987/0.9988

t/cZROG

432.2

382.2, 220.1

18.08 ± 0.02/18.77 ± 0.02

1.0

5×10−15–1×10−11

0.9992/0.9984

t/cZMP

514.2

220.1, 136.1

13.72 ± 0.02/14.67 ± 0.02

5.0

1×10−14–5×10−11

0.9990/0.9985

DHZ

222.1

136.1, 119.0

16.15 ± 0.04

0.1

5×10−16–1×10−11

0.9991

DHZR

354.2

222.1, 136.1

19.61 ± 0.01

0.05

1×10−16–5×10−10

0.9989

DHZ7G

384.2

222.1, 136.1

13.80 ± 0.02/14.13 ± 0.02

0.1

5×10−16–1×10−10

0.9994

DHZ9G

15.00 ± 0.01

0.05

1×10−16–1×10−10

0.9992

DHZOG

16.37 ± 0.03

0.1

5×10−16–5×10−12

0.9992

DHZROG

434.2

384.2, 222.1

19.22 ± 0.03

1.0

5×10−15–1×10−11

0.9983

DHZMP

516.2

222.1, 136.1

14.34 ± 0.01

1.0

5×10−15–1×10−11

0.9992

iP

204.1

136.1, 119.0

23.21 ± 0.01

0.1

5×10−16–1×10−11

0.9991

iPR

336.2

204.1, 136.1

23.88 ± 0.01

0.05

1×10−16–1×10−11

0.9989

iP7G

366.2

204.1, 136.1

18.70 ± 0.01

0.05

1×10−16–1×10−11

0.9988

iP9G

21.50 ± 0.01

0.5

1×10−15–1×10−11

0.9992

iPMP

416.2

204.1, 136.1

21.31 ± 0.02

5.0

1×10−14–5×10−11

0.9993

  1. a Values are means ± SD (n = 10); b Limit of detection, defined as a signal-to-noise ratio of 3:1.
  2. List of precursor and product ions of the studied compounds (the most abundant product ion is shown in bold type). The retention time stability, limits of detection, dynamic linear range, linearity (coefficient of determination, R2) are shown for UHPLC-ESI(+)-MS/MS analysis of isoprenoid cytokinins.