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Table 2 Method parameters

From: A new approach for cytokinin isolation from Arabidopsis tissues using miniaturized purification: pipette tip solid-phase extraction

Compounds Precursor Products Retention timea (min) LODb (fmol) Dynamic range (mol) R 2
t/cZ 220.1 136.1, 119.0 15.39 ± 0.04/16.82 ± 0.03 0.5 1×10−15–5×10−11 0.9989/0.9987
t/cZR 352.2 220.1, 136.1 19.06 ± 0.02/19.72 ± 0.01 0.1 5×10−16–5×10−11 0.9993/0.9986
tZ7G 382.2 220.1, 136.1 12.26 ± 0.02 0.1 5×10−16–1×10−11 0.9989
t/cZ9G 14.23 ± 0.02/15.13 ± 0.02 0.1 5×10−16–5×10−11 0.9993/0.9985
t/cZOG 14.83 ± 0.03/15.79 ± 0.03 0.5 1×10−16–1×10−11 0.9987/0.9988
t/cZROG 432.2 382.2, 220.1 18.08 ± 0.02/18.77 ± 0.02 1.0 5×10−15–1×10−11 0.9992/0.9984
t/cZMP 514.2 220.1, 136.1 13.72 ± 0.02/14.67 ± 0.02 5.0 1×10−14–5×10−11 0.9990/0.9985
DHZ 222.1 136.1, 119.0 16.15 ± 0.04 0.1 5×10−16–1×10−11 0.9991
DHZR 354.2 222.1, 136.1 19.61 ± 0.01 0.05 1×10−16–5×10−10 0.9989
DHZ7G 384.2 222.1, 136.1 13.80 ± 0.02/14.13 ± 0.02 0.1 5×10−16–1×10−10 0.9994
DHZ9G 15.00 ± 0.01 0.05 1×10−16–1×10−10 0.9992
DHZOG 16.37 ± 0.03 0.1 5×10−16–5×10−12 0.9992
DHZROG 434.2 384.2, 222.1 19.22 ± 0.03 1.0 5×10−15–1×10−11 0.9983
DHZMP 516.2 222.1, 136.1 14.34 ± 0.01 1.0 5×10−15–1×10−11 0.9992
iP 204.1 136.1, 119.0 23.21 ± 0.01 0.1 5×10−16–1×10−11 0.9991
iPR 336.2 204.1, 136.1 23.88 ± 0.01 0.05 1×10−16–1×10−11 0.9989
iP7G 366.2 204.1, 136.1 18.70 ± 0.01 0.05 1×10−16–1×10−11 0.9988
iP9G 21.50 ± 0.01 0.5 1×10−15–1×10−11 0.9992
iPMP 416.2 204.1, 136.1 21.31 ± 0.02 5.0 1×10−14–5×10−11 0.9993
  1. a Values are means ± SD (n = 10); b Limit of detection, defined as a signal-to-noise ratio of 3:1.
  2. List of precursor and product ions of the studied compounds (the most abundant product ion is shown in bold type). The retention time stability, limits of detection, dynamic linear range, linearity (coefficient of determination, R2) are shown for UHPLC-ESI(+)-MS/MS analysis of isoprenoid cytokinins.