Table 2 Effect of pre-treatment with opening buffer (OB) on seal lasting time of guard cells from all Arabidopsis lines Col-0, nia1nia2 , QC3, QL3, kc1-3, syp121, syp121ox, and dhar1-3
From: Protocol: optimised electrophyiological analysis of intact guard cells from Arabidopsis
| Â | Average of 8 lines | Col-0 | nia1nia2 | QC3 | ||||
|---|---|---|---|---|---|---|---|---|
Time (min) | Control | Pretreatment | Control | Pretreatment | Control | Pretreatment | Control | Pretreatment |
IK μA cm-2 experiments | 21.2±4.0 (179) | 34.4±3.8** (49) | 19.2±1.5 (72) | 36.8±3.1** (14) | 18.6±2.6 (7) | 33.7±2.7* (34) | 28.8±3.6 (45) | 40.0±6.8* (12) |
Ianion μA cm-2 experiments | 19.1±3.6 (45) | 35.0±4.6** (29) | 19.1±3.2 (12) | 28.3±4.9* (9) | 17.0±3.5 (15) | 37.5±5.2** (12) | 22.7±3.2 (13) | 52.0±14.2** (5) |