Effect of pre-treatment with opening buffer (OB) on I
in wild-type Arabidopsis . Steady-state current–voltage curves for Ianion from one guard cell recorded after 2-h pretreatment with OB. Current–voltage curves for Ianion are not corrected for background. Rundown in this cell was evident only after 65 min. Data shown are taken from voltage clamp scans at 10 (closed circles), 20 (open circles), and 30 (inverted closed triangles) and 40 min (open triangles) after the impalement. Conditioning voltage was +50 mV with 10-s steps to voltages between +50 mV and −220 mV. Inset: Raw current traces for recordings at 10, 20 and 30 min cross-referenced by symbol. Scale: vertical, 300 μA cm-2; horizontal, 5 s.