Multi-colour fluorescence detection in Poinsettia protoplasts. Poinsettia protoplasts were transformed with DNA constructs for ectopic expression of fluorescent proteins. Left: brightfield, middle: UV image, right: overlay. a) VIP1-YFP, b) VIP1-GFP, c) VIP1-CFP, d) YFP, e) mCherry. In images d) and e) also non-transformed protoplast(s) are shown to document the low level of nonspecific/background autofluorescence.