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Figure 1 | Plant Methods

Figure 1

From: Xylem sap collection and extraction methodologies to determine in vivo concentrations of ABA and its bound forms by gas chromatography-mass spectrometry (GC-MS)

Figure 1

Schematic outlining the separation of ABA, ABA-GE and Adducts I & II from xylem sap, and their release as ABA ex Adduct I, ABA ex Adduct II, ABA ex ABA-GE and free ABA, prior to subsequent derivatisation to pentafluoro-benzyl esters (PFB-ABA) (not shown but see text) for quantification by GC-MS. Horizontally paired rectangles represent key phase separations and are denoted as aqueous or organic accordingly. Abbreviations used: ABA, abscisic acid; ABA-GE, abscisic acid-glucose ester; D4ABA, tetra-deuteratedabscisic acid; CTMABr, cetyltrimethylammonium bromide; DCM, dichloromethane; IPA, iso-propanol.

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