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Figure 3 | Plant Methods

Figure 3

From: In planta assays involving epigenetically silenced genes reveal inhibition of cytosine methylation by genistein

Figure 3

Changes in frequency of methylcytosine in the CaMV 35S promoter as a consequence of treatment of C002 petunia plants with genistein. Sequencing data for PCR products amplified from bisulfite-treated DNA have been compiled. The height of the vertical lines shows the frequency of methylcytosine at respective positions per total PCR clones sequenced. Red, green, and blue lines indicate frequencies of methylcytosine at CpG, CpHpG, and CpHpH sites, respectively. For control and genistein-treated plants, 15 and 16 clones were sequenced, respectively. Numbers below the line indicate nucleotide positions relative to transcription start site of the CaMV 35S promoter. The analyzed sequences cover the -298 to -47 region of the promoter.

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