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Figure 1 | Plant Methods

Figure 1

From: Development of expression vectors based on pepino mosaic virus

Figure 1

Multiplication of PepMV CP-mutants in N. benthamiana protoplasts. (a) Schematic representation of PepXL6 and its derived CP mutants (PepXL6agg and PepGFPΔCP). RdRp-RNA-dependent RNA polymerase ORF; TG1, TGB2 and TGB3-triple gene block ORFs; CP-coat protein ORF. (b) Northern blot of total RNA prepared from N. benthamiana protoplasts 24 h post inoculation, hybridized with a replicase-specific RNA probe. Ethidium bromide-stained rRNA is shown (bottom panels).

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