Figure 1From: Development of expression vectors based on pepino mosaic virusMultiplication of PepMV CP-mutants in N. benthamiana protoplasts. (a) Schematic representation of PepXL6 and its derived CP mutants (PepXL6agg and PepGFPΔCP). RdRp-RNA-dependent RNA polymerase ORF; TG1, TGB2 and TGB3-triple gene block ORFs; CP-coat protein ORF. (b) Northern blot of total RNA prepared from N. benthamiana protoplasts 24 h post inoculation, hybridized with a replicase-specific RNA probe. Ethidium bromide-stained rRNA is shown (bottom panels).Back to article page