Figure 4

(A) Comparison of the incorporation of labeled nucleotides of rrn16 gene transcription over time in run-on (black line, y-axis on the left) and RePro (grey line, y-axis on the right) systems, measured after 2, 7, 15, 30, and 60 min of reaction time. (B) Decline of transcriptional activity in isolated chloroplasts. The amount of labeled rrn16 transcripts was analyzed in run-on (black line) and RePro (grey line) systems by pulsing the reaction by adding [³²P]-UTP during indicated intervals (0 - 10, 10 - 20, 20 - 30, 30 - 40, and 40 - 50 min). A schematic representation of the timing of [³²P]-UTP addition and RNA sampling is given below each graph.