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Figure 2 | Plant Methods

Figure 2

From: A novel Gateway®-compatible binary vector allows direct selection of recombinant clones in Agrobacterium tumefaciens

Figure 2

The growth of Agrobacterium tumefaciens strain on LB agar plates with different selections. A. tumefaciens strain GV2260 grew equally well on LB medium with (A) or without 5% sucrose (B). The ccdB gene cassette in the pEarleyGate 101 vector could not inhibit the growth of A. tumefaciens on LB medium with (C) or without 5% sucrose (D). A. tumefaciens strain GV2260 carrying the newly generated pEG101-SacB/R destination vector grew well on LB medium without sucrose (E), but growth was inhibited on LB medium supplemented with 5% sucrose (F).

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