From: A simple and efficient method for isolating small RNAs from different plant species
Tissue | Modification | ng/μl | Average 260/280 | Average 260/230 | Yield (μg) |
---|---|---|---|---|---|
Cactus | 1) LMW RNA enrichment from the supernatants obtained after precipitation of total RNA | 382.30 | 1.82 | 1.81 | 7.65 |
2) LiCl precipitating (overnight), followed by LMW RNA enrichment | 527.75 | 1.81 | 1.90 | 10.56 | |
3) Addition of LiCl, directly followed by LMW RNA enrichment | 291.42 | 1.66 | 1.35 | 5.83 | |
4) Enrichment of LMW RNA directly after the chloroform extraction without addition of LiCl | 1008.42 | 2.02 | 1.04 | 20.17 | |
Arabidopsis | 1) LMW RNA enrichment from the supernatants obtained after precipitation of total RNA | 582.52 | 1.68 | 1.23 | 11.65 |
2) LiCl precipitating (overnight), followed by LMW RNA enrichment | 333.68 | 1.66 | 1.21 | 6.67 | |
3) Addition of LiCl, directly followed by LMW RNA enrichment | 609.38 | 1.53 | 1.13 | 12.19 | |
4) Enrichment of LMW RNA directly after the chloroform extraction without addition of LiCl | 1418.77 | 1.53 | 0.96 | 28.38 |