Table 3 Description of six primer sets tested and the resultant PCR products
From: Universal endogenous gene controls for bisulphite conversion in analysis of plant DNA methylation
Primer pair | Forward primer sequence (5'→3') | Reverse primer sequence (5'→3') | Tm (°C) | Expected size (bp) | No. of cytosine in target region |
|---|---|---|---|---|---|
IND.a-A3 | GGAGGAGGAGAGGAAGYAGAAGAA | CCTRRCACCATCCTCTTCAATATCC | 58, 58 | 239 | 43 |
ATP1-1 | TGAAYGAGATTYAAGYTGGGGAAATGGT | CCCTCTTCCATCAATARRTACTCCCA | 50, 56 | 227 | 64 |
ATP1-2 | TAGTAAAYAGGYGGTGGYATATYGA | CTCTRTTTCCAAACARATTTRTCCATC | 50, 56 | 252 | 15 |
NAD | AGTTTYTGYTAGAYGAGAAATAAGGA | CCTACTCACTCRRACAATRCTCT | 50, 56 | 276 | 24 |
ATS1-P1 | AGGTTYAGGGTTTTGGTAGTGAGAAGGGA | TCCATRACAATCCTAACAACAATTATCA | 51, 54 | 305 | 54-58 |
ATS1-P2 | TGGAGGAGYAGAGGYGAAGYTTGA | ACCAARACCCRCCACAACACATRCCT | 55, 64 | 227 | 24-28 |