Figure 2

Selection and regeneration of P. aegyptiaca. A) P. aegyptiaca clones with parasitic infective roots ready to be inoculated on tomato. B) Sucessful P. aegyptiaca explants infecting tomato host root in a minirhizotron system, demonstrating that the parasite is able to regenerate its whole plant structure, including anchorage roots and shoots. Black explants were not able to infect the tomato roots. C) Wild type P. aegyptiaca callus growing in MS media without kanamycin. D) Wild type P. aegyptiaca callus growing in MS media suplemented with 100 μg/ml of kanamycin. E) Wild type P. aegyptiaca callus observed under white light and F) UV illuminated image of E, showing only slight autofluorescence from the seed coat.