Co-localization analysis with known organelle markers. A, Plastid-YFP and plastid-mCherry markers, or B, Golgi-YFP and plastid-mCherry markers were co-expressed in rice green tissue protoplasts. Cells showing both markers in each co-transfection are indicated by arrowheads in the merged images of YFP, mCherry and chlorophyll autofluorescence (Chl) signals. Bright field images of protoplasts are also shown. Scale bar = 20 μm.