Oligonucleotide properties
|
Melting temperature = 70°C (+/- 8°C)
|
97.18%
|
T
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Nucleotide composition: oligonucleotide cannot have a contiguous single nucleotide base repeat longer than 8 bases AND probe GC content fits the 20-to-60% range AND each base cannot constitute more than 40% of the oligonucleotide sequence
|
85.33%
|
C
|
Secondary structure
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Stem of potential hairpin structure cannot be longer than 8 bases
|
99.20%
|
H
|
Probe specificity
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Minimum Hamming distance to non-target parts of genome > 25
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99.45%
|
D
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Substring: not more than 20 contiguous bases common to other (non-target) parts of genome
|
99.02%
|
S
|