|
Oligonucleotide properties
|
|
Melting temperature = 70°C (+/- 8°C)
|
97.18%
|
T
|
|
Nucleotide composition: oligonucleotide cannot have a contiguous single nucleotide base repeat longer than 8 bases AND probe GC content fits the 20-to-60% range AND each base cannot constitute more than 40% of the oligonucleotide sequence
|
85.33%
|
C
|
|
Secondary structure
|
|
Stem of potential hairpin structure cannot be longer than 8 bases
|
99.20%
|
H
|
|
Probe specificity
|
|
Minimum Hamming distance to non-target parts of genome > 25
|
99.45%
|
D
|
|
Substring: not more than 20 contiguous bases common to other (non-target) parts of genome
|
99.02%
|
S
|
