Presentation of the selected parameters in the final microarray probe set. A. Comparison of numbers of probes not meeting each design criterion (T - Melting Temperature, D - Hamming Distance, S - Substring, H - Hairpin, C - Nucleotide Composition, 1 type (2 types, 3 types, 4 types) - probes for which 1 criterion (2, 3 or 4 criteria, respectively) are not fulfilled, Any - probes that do not meet at least one design criterion). B. Frequency distribution of probe GC content (a component of the Nucleotide Composition criterion). C. Frequency distribution of probe melting temperatures. D. Frequency distribution of minimum Hamming distance of the probes, describing the probe specificity within the cucumber chloroplast genome.