Figure 5

Diagram of rapid sample preparation for SDS-PAGE and genomic PCR analysis from Arabidopsis. The lysis solution for SDS-PAGE was prepared by mixing stock solutions of its components (0.5 M EDTA, pH 8.0, 1 M Tris-HCl, pH 6.8, 10% w/v SDS, 100% β-ME, 100% glycerol and bromophenol blue powder). The pH value of this solution was around 7.4. The lysis solution for genomic PCR was prepared in the same way. Arabidopsis cells can be rapidly lysed by boiling plants in the indicated solutions for 5-10 minutes (until the solution become cloudy by cell extracts). In the case of SDS-PAGE, the solution can be directly loaded onto the gel after boiling. In the case of genomic PCR, the solution is diluted after boiling (500 μl distilled water for 50 μl lysis solution) and usable as a PCR template.