Figure 3
From: sunTILL: a TILLING resource for gene function analysis in sunflower
Cel I-nuclease mismatch cleavage assay and evaluation of pool capacity for SNP detection. (A) The IRDy671 image represents reactions carried out for three different digestion times (15, 30 and 45 minutes) with progressive enzyme dilutions (1:2, 1:4, 1:10 and 1:20). The expected identified SNP is highlighted with a red rectangle. The first lane is represented by 50-700 bp Sizing Standard (LiCOR). (B) Evaluation of pool capacity for Cel I SNP detection. From left to right of the IRDy671 channel image, in the first lane the sizing standard is represented; second and third lanes respectively represent single DNAs from XRQ and PSC8 genotypes as negative controls; the 2-, 4-, 8-, 12-, 16- and 20-fold pools are shown in the subsequent six lanes.