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Figure 3 | Plant Methods

Figure 3

From: A novel procedure for the quantitative analysis of metabolites, storage products and transcripts of laser microdissected seed tissues of Brassica napus

Figure 3

Total starch in laser-microdissected tissues of B. napus as measured by ion chromatography. (a &b) Effect of different HCl treatments on hydrolysis of starch and cellulose standards. (c) Level of starch detected in seed cross sections following either amyloglucosidase or HCl-mediated hydrolysis of starch. (d) Starch detected in dissected samples of 1 element per tissue type (minimum tissue amount ~ 0.0063 mm3). (e) Starch staining on a fixed seed cross section. Dark spots indicate accumulation of starch. (f) Laser dissected tissues showing hypocotyl (hy), inner cotyledon (ic) and outer cotyledon (oc). Mean values +/- standard deviation are shown (n = 5) in a-d. Stars in d indicate statistically significant differences versus hypocotyl according to t-test (p < 0.01).

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Plant Methods

ISSN: 1746-4811