Skip to main content
Figure 2 | Plant Methods

Figure 2

From: Protocol: An updated integrated methodology for analysis of metabolites and enzyme activities of ethylene biosynthesis

Figure 2

Schematic overview of the ACC and MACC protocol (protocol 2). Following extraction of tissue with SSA, the extract is divided into two aliquots. The first aliquot is used for the determination of the free ACC content of the sample, while the second is first hydrolysed, before the total ACC content of the sample is measured. The MACC concentration of the sample is calculated by subtracting the free ACC concentration from the total ACC concentration.

Back to article page

Plant Methods

ISSN: 1746-4811