Figure 5
From: An effective virus-based gene silencing method for functional genomics studies in common bean
VIGS of PvSACPD in common bean plants by the BPMV-RNA2 vector using two BPMV strains as sources of RNA1. Variation in SACPD-C' transcript relative amounts in common bean plants inoculated with treatments: HopV (RNA1 from strain K-Hop plus empty vector), HanV (RNA1 from strain K-Han plus empty vector), HopD and HanD, RNA1 from strains H-Hop and K-Han, respectively, plus recombinant RNA2-SACPD (D). Black solid bars, SACPD-C' expression levels in treated plants 15 dpi, stripped bars, SACPD-C' expression levels in treated plants 25 dpi. Bars represent the mean value of three biological and technical replicates. Error bars represent standard error. P-values < 0.01. (Right panel). Silencing of common bean SACPD s induced a chlorotic/necrotic phenotype, as previously shown in soybean plants [13].