Agrobacterium-mediated transformation of safflower and the efficient recovery of transgenic plants via grafting

Table 3 Survival of transgenic shoots and plants through critical steps of the transformation protocol

Genotype	Cotyledons co-cultivated with *Agrobacterium*^a	Shoots ready to graft obtained^b	Successful grafts^c	Mature plants obtained^d	Final transformation efficiency^e
S-317	1206	115	58	58	4.8%
WT	1660	95	52	52	3.1%
combined	2866	210	110	110	3.8%

^a Summary of a total of 20 independent experiments
^b Shoots are positive for GFP and at least 3 cm in length
^c Successful grafts as judged by new leaf growth within 2 weeks of grafting, yet still growing in controlled tissue culture conditions
^d Mature plants grown in soil and in a glasshouse
^e Transformation efficiency calculated as the (mature plants/infected cotyledons)x100

ISSN: 1746-4811