Agrobacterium-mediated transformation of safflower and the efficient recovery of transgenic plants via grafting

Table 1 Composition of media for transformation of safflower from cotyledon explants.

Component	S₁ Co-cultivation		S₂ Callus induction		S₃ Shoot initiation		S₄ Shoot out-growth		S₅ Shoot elongation
	S-317	WT	S-317	WT	S-317	WT	S-317	WT	S-317	WT
Media	MS[33]	MS	MS	MS	MS	MS	MS	MS	MS	MS
Growth regulators
TDZ	-	1	-	1	-	-	-	-	-	-
NAA	0.1	0.1	0.1	0.1	-	-	-	-	-	-
BA	1	-	1	-	1	-	-	-	-	-
KN	-	-	-	-	-	1	-	-	-	-
2iP	-	-	-	-	-	-	-	0.5	-	0.5
GA	-	-	-	-	-	-	-	-	0.1	0.1
Antioxidants
L-cysteine	50	50	-	-	-	-	-	-	-	-
Ascorbic acid	15	15	-	-	-	-	-	-	-	-
PVP	-	-	-	-	-	-	-	-	500	500
Antibiotics
Hygromycin	-	-	20	20	20	20	18	18	-	-
Cefotaxime	-	-	250	250	250	250	250	250	250	250
Timentin Others	-	-	50	50	50	50	50	50	50	50
Iota- carrageenan	-	-	1500	1500	1500	1500	1500	1500	-	-
AgNo₃	-	-	3	3	3	3	1.5	1.5	1.5	1.5
Sucrose	30000	30000	30000	30000	30000	30000	20000	20000	20000	20000
Agar	9000	9000	9000	9000	9000	9000	-	-	-	-
Phytagel	-	-	-	-	-	-	5000	5000	5000	5000

Amounts indicate the mg l^-1 of each component. S-317 and WT refer to high oleic acid content seeds and high linoleic acid content seeds, respectively.

ISSN: 1746-4811