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Table 4 Method overview.

From: Proposal for field sampling of plants and processing in the lab for environmental metabolic fingerprinting

Step in processing

Most suitable result with:

Pre-treatment

processing of fresh plant material

Grinding

handheld disperser, speed of 15,000 min-1

Extraction: solvent mixture

2:1 methanol:dichloromethane

Extraction: efficiency

threefold extraction:

 

1. extraction step at untreated pH 6,

 

2. extraction step pH 2,

 

3. extraction step pH 9

Extraction: influence of time

first extraction step for one week

Extraction: influence of temperature

ambient temperature

Shaking and phase separation

shaking after the extracts of all three extraction

 

steps were pooled

Storage of samples

storage of completely processed extracts until analysis at 4°C

  1. Representation of most suitable method (highest reproducibility and handling comfort) for every processing step for metabolic fingerprinting of plant material (Plantago lanceolata) sampled in the field.
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Plant Methods

ISSN: 1746-4811