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Table 1 Comparison of experimental steps and durations for EdU-assay and BrdU-based immunolocalization

From: A rapid and robust assay for detection of S-phase cell cycle progression in plant cells and tissues by using ethynyl deoxyuridine

  EdU assay BrdU immunolocalization
EdU or BrdU labeling 2 h* 2 h*
Fixation and washes 15 min + 15 min 15 min + 15 min
Cell wall digestion and washes - 30 min + 15 min
Plasma membrane permeabilization and washes - 30 min + 15 min
DNase I digestion and washes - 30 min + 15 min
EdU click reaction or BrdU antibody incubations 30 min 3 h + 25 min + 1 h
Washing with DAPI and sample mounting 20 min 20 min
Total experimental duration 3 h 20 min 9 h 30 min
  1. * Depending on the proliferation activity of a given cell type, shorter incubations may be sufficient or longer incubations may be necessary.