Figure 6From: A rapid and robust assay for detection of S-phase cell cycle progression in plant cells and tissues by using ethynyl deoxyuridineQuantitative analysis of EdU incorporation on rice root meristems using EdU assay. Rice roots (O. sativa L. ssp. japonica 'Nipponbare') were incubated for 2, 4, 6 hours with 20 μM EdU which was detected using Alexa Fluor 488 azide (green). (A) Fluorescence stereo microscopy images of EdU-labeled root tips. Transmission images of roots were pseudocolored red and merged with green EdU signal. Scalebar 300 μm. (B) Laser scanning confocal microscopy images of single optical sections of 6 μm (optical depth) on the median plane of rice root tips. Cell wall autofluorescence (red pseudocolor) and transmission images were overlaid onto green EdU signal. Inset photo shows a close-up of a 4 h EdU-treated cell with EdU-Alexa Fluor 488 labeled segregating chromosomes. Scalebar 300 μm. (C) Confocal images of EdU-labeled and DAPI-stained (DNA) cells from the meristem region of 2 h EdU treated root tips. Arrowheads (from left to right) indicate an anaphase, a prophase and a metaphase cell with well-preserved morphology. Scalebar 10 μm. (D) Quantitation of average EdU signal intensity of the meristem region after 2, 4 and 6 hours EdU incubation.Back to article page