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Table 1 Comparison of different DNA isolation protocols

From: NEATTILL: A simplified procedure for nucleic acid extraction from arrayed tissue for TILLING and other high-throughput reverse genetic applications

Protocol

Feasibility of isolation in 96 well plates

Yield (μg/100 mg tissue)

A260/280 ratio

Time required (h)

Cost (in USD/100 samples)*

Krysan et al[23]

Yes

Nil

Nil

1

6

Kang and Yang[24]

No

10

1.12-1.37

1-3

10-20

Chao & Somers[25]

Yes

15

1.9

3

9

CTAB[21]

No

6.5

1.89

4-5

12

DNAzol[22]

No

3.5

1.16

3

164

Qiagen[26]

Yes

5

1.63

2

364

NEATTILL

Yes

40

1.83

7

14

  1. *The indicated cost is for 100 DNA preparations using Eppendorf tubes. DNA isolation with 96 deepwell plate using NEATTILL costs 40 USD/plate compared to 220 USD/plate with Qiagen protocol.
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Plant Methods

ISSN: 1746-4811