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Figure 3 | Plant Methods

Figure 3

From: Normalization using ploidy and genomic DNA copy number allows absolute quantification of transcripts, proteins and metabolites in cells

Figure 3

Analysis of RBC-L and LHCP accumulation in A. thaliana. (A) Total protein extract prepared from 3-week-old wild-type (odd-numbered lanes) and abc4 (even-numbered lanes) plants was subjected to SDS-PAGE analysis followed by CBB staining; sample amounts varied according to the normalization factor indicated at top. Lanes 1 and 2, 20.0 μg total protein; lanes 3 (17.9 μg total protein) and 4 (13.5 μg total protein) correspond to 1.5 mg of fresh weight; lanes 5 (18.9 μg total protein) and 6 (1.2 μg total protein) correspond to 10% of the material from an individual plant (wild type: 1.55 mg of fresh weight; abc4: 0.35 mg of fresh weight); lanes 7 (20.0 μg total protein) and 8 (12.4 μg total protein) correspond to equivalent cell numbers of wild-type and mutant samples. (B) Quantification of RBC-L and LHCP accumulation in wild-type and abc4 plants. The density of each CBB-stained protein band in panel (A) was quantified using ImageJ http://rsbweb.nih.gov/ij/. Data reflect the mean ± standard deviation (n = 4) relative to the indicated normalization factor.

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Plant Methods

ISSN: 1746-4811