Table 1 Primers used for quantitative real time PCR.
Target | Primer sequences (designed using Primer 3 [26]) |
|---|---|
18S: U43011 | Fw: 5'-GACTACGTCCCTGCCCTTTG (nt 1571-1590) Rev: 5'- AACACTTCACCGGACCATTCA (nt 1618-1638) |
PsSym19: AF491997 | Fw: 5'- GATTGGTTGTGGCGTGTGTTC (nt 317-337) Rev: 5'- GCTCTCAAACCCTTCAGTTGC (nt 373-393) |
PsPT4: GU721059 | Fw:5'-CAATATTGTCACCGGTGTGGCACTTGTC (nt 197-224) Rev: 5' GGAAGAACCGAATGAAAGGCCGGAGCA (nt 327-353) |