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Figure 4 | Plant Methods

Figure 4

From: A simple, rapid method to isolate salt glands for three-dimensional visualization, fluorescence imaging and cytological studies

Figure 4

Isolated salt glands of A . officinalis showing their intrinsic autofluorescent nature. (A-C) Side view of salt gland. Note the presence of 2 secretory cells (sc) above the stalk cell (st). (D-F) Top view of salt gland with a total of 7 secretory cells (green patches) observed. The outlines of these cells were emphasized by their autofluorescent nature. Excitation wavelength was 488 nm (8-10%) and optical sections were taken using Zeiss LSM 510. Images from optical sectioning were stacked to give a clearer picture of the salt glands. Bars = 5 μm.

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Plant Methods

ISSN: 1746-4811