Figure 10
Cytological studies of isolated salt glands of A . officinalis with the use of various fluorescent dyes. (A) Top view of isolated salt gland treated with Coulter® DNA-Prep Stain (containing 8 μg ml-1 PI). (B, C) Confocal microscopic images of isolated salt glands after LysoTracker® Red DND-99 (40 nM) staining. Note the relatively large (yellow arrows) and small (red arrows) internal cellular structures, which are presumably to be organelles that are acidic in nature. Prominent unstained regions (*) are observed especially in the secretory cells of (B). Non-specific staining of the cuticular envelope (white arrows) is also observed. (D, E) Confocal microscopic images of isolated salt glands stained with 6.6 nM FM®4-64. Intense staining of spherical-like structures (arrows) is detected within the secretory cells. Insets: corresponding image of the same salt gland captured simultaneously in the fluorescent and transmission modes. (F) Top view of isolated salt gland showing apparent staining of cells (*) after incubation in MitoTracker® Red CM-H2XRos. Inset: corresponding bright-field image of (F) taken together with overlapping image recorded simultaneously in the transmission and fluorescent modes. Side view (G) and top view (H, I) of isolated salt glands stained with Texas Red®-X phalloidin showing the presence of actin-like filaments (arrows). (I) An enlarged portion of (H) with prominent actin-like filaments (arrows) along the peripheral regions. Bars = 5 μm.