Table 1 Isolation of embedded Eucalyptus secretory cavities via enzymatic digestion.
From: Isolation of intact sub-dermal secretory cavities from Eucalyptus
Enzyme (Manufacturer) | Source | Time (h)‡ | Quality of isolation | Comments |
|---|---|---|---|---|
Pectinase P-4716 (Sigma) | Aspergillus niger | 12 | Excellent | Successful isolation of cavities free from mesophyll cells. Mesophyll cells remain green. |
Pectinase PASE (Worthington) | Aspergillus niger | 16 | Good | Mostly successful isolation of cavities free from mesophyll cells. Mesophyll cells become brown. |
Pectinase P-2401 (Sigma) | Rhizopus spp. | 24 | Poor | Mesophyll cells generally remain attached to cavities. Mesophyll cells become brown. |
Macerase-pectinase 'Calbiochem' (Merck) | Rhizopus spp. | 24 | Poor | Mesophyll cells generally remain attached to cavities. Mesophyll cells become brown. |
Cellulase C-1794 (Sigma) | Trichoderma viride | 24 | Poor | Cavities lose structural integrity. Lumen contents become brown. Mesophyll cells become brown. |
Cellulase 'Onozuka RS' (Yakult) | Trichoderma viride | 24 | Poor | Cavities lose structural integrity. Lumen contents become yellow. Mesophyll cells become brown. |