A high-density Diversity Arrays Technology (DArT) microarray for genome-wide genotyping in Eucalyptus

Table 6 Four additional genomic representation libraries and corresponding numbers of clones used for the development of the interim and operational DArT microarray

No. of clones in library	No. of individuals	Species	Source	RE digestion
1920 *	16	E. grandis × E. urophylla (IP pedigree)	Brazil	Pst I/Taq I
	16	E. grandis × E. urophylla (VCP pedigree)	Brazil	Pst I/Taq I
	16	E. camaldulensis × (E. urophylla × E. globulus)	Brazil	Pst I/Taq I
	16	(E. grandis × E. urophylla) × (E. urophylla × E. globulus)	Brazil	Pst I/Taq I
	16	(E. dunnii × E. grandis) × (E. urophylla × E. globulus)	Brazil	Pst I/Taq I
	16	(E. dunnii × E. grandis) × E. urophylla	Brazil	Pst I/Taq I
1152 **	1	E. grandis (BRASUZ1)	Brazil	Pst I/Taq I
2304 ***	62	Several species	Australia	Pst I/Taq I
2304 ***	62	Several species	Australia	Pst I

* Library built by pooling equimolar quantities of DNA of 96 inter-specific hybrids.
** Library built with DNA of E. grandis tree BRASUZ1, whose full genome is being sequenced.
*** The following species were used: E. albens, E. balladoniensis, E. bicostata, E. biterranea, E. brassiana, E. brevistylis, E. camaldulensis, E. cladocalyx, E. coolabah, E. cordata, E. cornuta, E. cosmophylla, E. crebra, E. dalrympleana, E. deglupta, E. delicata, E. diversicolor, E. dundasii, E. dunnii, E. falcata, E. glaucescens, E. glaucina, E. globulus, E. gomphocephala, E. grandis, E. gunnii, E. hallii, E. houseana, E. howittiana, E. leucophloia, E. lockyeri, E. longifolia, E. lucasii, E. maidenii, E. michaeliana, E. microcorys, E. morrisbyi, E. nitens, E. obtusiflora, E. optima, E. ovata, E. pachycalyx, E. pachyphylla, E. paludicola, E. perriniana, E. platyphylla, E. polyanthemos, E. populnea, E. pseudoglobulus, E. pulverulenta, E. pumila, E. ravereana, E. rubida, E. salmonophloia, E. scoparia, E. stoatei, E. tereticornis, E. torquata, E. urophylla, E. viminalis, E. wandoo, E. woodwardii.

ISSN: 1746-4811