Figure 2

Overview of pUCE vectors. A USER_TC-TG site was introduced in the plasmid pWBVec8 to generate pUCE as described in the text. pUCE_{D-Hord:HvHB1:NOS} was generated from pUCE in a single step by combining PCR products of D-Hordein promoter, HvHB1 cDNA and NOS. pUCE_{UBI:GFP-ATG8:NOS} was generated from pUCE_UBI:USER:NOS in a single step by combining PCR products of eGFP and ATG8. The remaining vectors were generated by insertion of single PCR products. A USER™ site is lost when a PCR product is inserted. However, it can be reconstituted if included in one of the primers of the PCR reaction.