From: Protocol: a simple gel-free method for SNP genotyping using allele-specific primers in rice and other plant species
Mismatching base
A
T
G
C
Matching base
0%
50%
60%
(0/10)
(5/10)
(6/10)
90%
10%
(9/10)
(1/10)
40%
(4/10)