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Table 2 Quantification of Picolinyl ester derivatives of N. attenuata leaf extracts and comparison with two additional analytical methods.

From: A rapid and sensitive method for the simultaneous analysis of aliphatic and polar molecules containing free carboxyl groups in plant extracts by LC-MS/MS

    Treatments
    0 min 30 min 60 min
      wound FAC wound FAC
Substance RT [M+H] + Picol. esters Method 2 or 3* Picol. esters Method 2 or 3* Picol. esters Method 2 or 3* Picol. esters Method 2 or 3* Picol. esters Method 2 or 3*
    ng gFW -1 (± SE) ng gFW -1 (± SE) ng gFW -1 (± SE) ng gFW -1 (± SE) ng gFW -1 (± SE) ng gFW -1 (± SE) ng gFW -1 (± SE) ng gFW -1 (± SE) ng gFW -1 (± SE) ng gFW -1 (± SE)
JA-Ile 6.86 415 - 1.1
(± 0.14)
39
(± 4.0)
46
(± 4.8)
92
(± 6.0)
81
(± 2.0)
83
(± 9.0)
97
(± 4.0)
158
(± 16)
163
(± 19)
SA 8.77 230 193
(± 31)
173
(± 23)
203
(± 24)
212
(± 19)
182
(± 15)
196
(± 11)
216
(± 22)
189
(± 17)
116
(± 18)
129
(± 22)
ABA 9.01 356 257
(± 7)
225
(± 8.0)
192
(± 35)
246
(± 9.0)
274
(± 16)
232
(± 14)
264
(± 6.0)
242
(± 10)
277
(± 12)
270
(± 14)
ICA 9.08 253 - - - - - - - - - -
CA 9.34 240 - - - - - - - - - -
JA 9.43 302 137
(± 20)
152
(± 23)
937
(± 150)
845
(± 95)
1845
(± 125)
1586
(± 110)
1564
(± 180)
1326
(± 42)
3820
(± 350)
3149
(± 223)
Traumatol 9.85 306 - - - - - - - - - -
dnOPDA 9.85 356 - - - - - - - - - -
IAA 9.94 267 - - - - - - - - - -
Traumatin 9.96 304 11.3
(± 5.6)
14.4
(± 7.8)
17.1
(± 4.8)
15.2
(± 2.3)
14.6
(± 3.4)
12.4
(± 2.7)
13.8
(± 1.9)
15
(± 5.2)
10.7
(± 3.6)
12.2
(± 1.6)
Tr. acid** 10.15 320 - 5
(± 0.96)
19.6
(± 3.2)
24.3
(± 6.7)
23.8
(± 6.1)
20.4
(± 4.8)
17.1
(± 2.5)
19.6
(± 2.3)
20.9
(± 1.9)
21.4
(± 4.2)
OPC4:0 10.46 330 - - - - 12.6
(± 8.1)
- 10.3
(± 4.9)
- 18.5
(± 4.7)
-
OPC6:0 10.67 358 - - - - 5.3
(± 2.9)
- 8.6
(± 3.5)
- 11.2
(± 5.6)
-
OPDA 10.89 384 107
(± 60)
91
(± 53)
323
(± 47)
386
(± 45)
291
(± 11)
322
(± 15)
180
(± 45)
184
(± 15)
301
(± 31)
313
(± 3.0)
GA3 11.27 438 - - - - - - - - - -
OPC8:0 11.41 386 - - - - - - - - - -
GA4 11.99 424 - - - - - - - - - -
18:3 12.83 370 446
(± 38)
398
(± 24)
480
(± 53)
497
(± 37)
523
(± 30)
497
(± 42)
605
(± 20)
596
(± 31)
476
(± 33)
424
(± 31)
16:1 13.2 346 52
(± 9)
26
(± 23)
41
(± 3.0)
22
(± 20)
48
(± 12)
51
(± 32)
91
(± 26)
147
(± 96)
103
(± 13)
121
(± 83)
16:2 10.93 344 - - - - - - - - - -
16:3 10.29 342 45.6
(± 12)
- 41.1
(± 2.0)
28.2
(± 23.0)
64.1
(± 19)
40.5
(± 21)
130.2
(± 10)
80.9
(± 39)
37.2
(± 3.0)
-
18:2 14.22 372 245
(± 16)
190
(± 17)
251
(± 8.0)
189
(± 13)
316
(± 45)
398
(± 23)
377
(± 43)
415
(± 26)
333
(± 209)
367
(± 25)
16:0 14.53 348 21100
(± 3000)
22300
(± 4150)
16600
(± 930)
14100
(± 2300)
18600
(± 2800)
15400
(± 4700)
23200
(± 3200)
21600
(± 3000)
14600
(± 900)
15600
(± 3300)
18:1 14.86 374 625
(± 47)
502
(± 66)
603
(± 22)
572
(± 72)
805
(± 59)
716
(± 28)
791
(± 84)
778
(± 47)
894
(± 36)
813
(± 51)
18:0 17.59 376 46800
(± 12900)
44600
(± 7300)
47900
(± 1200)
33400
(± 9300)
49000
(± 5100)
36700
(± 4600)
45700
(± 6200)
41300
(± 3300)
39400
(± 5300)
33000
(± 2700)
  1. Quantification of compounds was performed in triplicate (n = 3)
  2. -: below limit of detection under the conditions tested
  3. * Method 2: Analysis of underivatized molecules by LC-MS/MS, this method was used to quantify compounds in the upper list section
  4. * Method 3: Analysis of methyl esters of FFA (FAMES) by GC-MS
  5. ** Tr. acid: Traumatic acid