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Table 2 Quantification of Picolinyl ester derivatives of N. attenuata leaf extracts and comparison with two additional analytical methods.

From: A rapid and sensitive method for the simultaneous analysis of aliphatic and polar molecules containing free carboxyl groups in plant extracts by LC-MS/MS

    Treatments
    0 min 30 min 60 min
      wound FAC wound FAC
Substance RT [M+H] + Picol. esters Method 2 or 3* Picol. esters Method 2 or 3* Picol. esters Method 2 or 3* Picol. esters Method 2 or 3* Picol. esters Method 2 or 3*
    ng gFW -1 (± SE) ng gFW -1 (± SE) ng gFW -1 (± SE) ng gFW -1 (± SE) ng gFW -1 (± SE) ng gFW -1 (± SE) ng gFW -1 (± SE) ng gFW -1 (± SE) ng gFW -1 (± SE) ng gFW -1 (± SE)
JA-Ile 6.86 415 - 1.1
(± 0.14) 		39
(± 4.0) 		46
(± 4.8) 		92
(± 6.0) 		81
(± 2.0) 		83
(± 9.0) 		97
(± 4.0) 		158
(± 16) 		163
(± 19)
SA 8.77 230 193
(± 31) 		173
(± 23) 		203
(± 24) 		212
(± 19) 		182
(± 15) 		196
(± 11) 		216
(± 22) 		189
(± 17) 		116
(± 18) 		129
(± 22)
ABA 9.01 356 257
(± 7) 		225
(± 8.0) 		192
(± 35) 		246
(± 9.0) 		274
(± 16) 		232
(± 14) 		264
(± 6.0) 		242
(± 10) 		277
(± 12) 		270
(± 14)
ICA 9.08 253 - - - - - - - - - -
CA 9.34 240 - - - - - - - - - -
JA 9.43 302 137
(± 20) 		152
(± 23) 		937
(± 150) 		845
(± 95) 		1845
(± 125) 		1586
(± 110) 		1564
(± 180) 		1326
(± 42) 		3820
(± 350) 		3149
(± 223)
Traumatol 9.85 306 - - - - - - - - - -
dnOPDA 9.85 356 - - - - - - - - - -
IAA 9.94 267 - - - - - - - - - -
Traumatin 9.96 304 11.3
(± 5.6) 		14.4
(± 7.8) 		17.1
(± 4.8) 		15.2
(± 2.3) 		14.6
(± 3.4) 		12.4
(± 2.7) 		13.8
(± 1.9) 		15
(± 5.2) 		10.7
(± 3.6) 		12.2
(± 1.6)
Tr. acid** 10.15 320 - 5
(± 0.96) 		19.6
(± 3.2) 		24.3
(± 6.7) 		23.8
(± 6.1) 		20.4
(± 4.8) 		17.1
(± 2.5) 		19.6
(± 2.3) 		20.9
(± 1.9) 		21.4
(± 4.2)
OPC4:0 10.46 330 - - - - 12.6
(± 8.1) 		- 10.3
(± 4.9) 		- 18.5
(± 4.7) 		-
OPC6:0 10.67 358 - - - - 5.3
(± 2.9) 		- 8.6
(± 3.5) 		- 11.2
(± 5.6) 		-
OPDA 10.89 384 107
(± 60) 		91
(± 53) 		323
(± 47) 		386
(± 45) 		291
(± 11) 		322
(± 15) 		180
(± 45) 		184
(± 15) 		301
(± 31) 		313
(± 3.0)
GA3 11.27 438 - - - - - - - - - -
OPC8:0 11.41 386 - - - - - - - - - -
GA4 11.99 424 - - - - - - - - - -
18:3 12.83 370 446
(± 38) 		398
(± 24) 		480
(± 53) 		497
(± 37) 		523
(± 30) 		497
(± 42) 		605
(± 20) 		596
(± 31) 		476
(± 33) 		424
(± 31)
16:1 13.2 346 52
(± 9) 		26
(± 23) 		41
(± 3.0) 		22
(± 20) 		48
(± 12) 		51
(± 32) 		91
(± 26) 		147
(± 96) 		103
(± 13) 		121
(± 83)
16:2 10.93 344 - - - - - - - - - -
16:3 10.29 342 45.6
(± 12) 		- 41.1
(± 2.0) 		28.2
(± 23.0) 		64.1
(± 19) 		40.5
(± 21) 		130.2
(± 10) 		80.9
(± 39) 		37.2
(± 3.0) 		-
18:2 14.22 372 245
(± 16) 		190
(± 17) 		251
(± 8.0) 		189
(± 13) 		316
(± 45) 		398
(± 23) 		377
(± 43) 		415
(± 26) 		333
(± 209) 		367
(± 25)
16:0 14.53 348 21100
(± 3000) 		22300
(± 4150) 		16600
(± 930) 		14100
(± 2300) 		18600
(± 2800) 		15400
(± 4700) 		23200
(± 3200) 		21600
(± 3000) 		14600
(± 900) 		15600
(± 3300)
18:1 14.86 374 625
(± 47) 		502
(± 66) 		603
(± 22) 		572
(± 72) 		805
(± 59) 		716
(± 28) 		791
(± 84) 		778
(± 47) 		894
(± 36) 		813
(± 51)
18:0 17.59 376 46800
(± 12900) 		44600
(± 7300) 		47900
(± 1200) 		33400
(± 9300) 		49000
(± 5100) 		36700
(± 4600) 		45700
(± 6200) 		41300
(± 3300) 		39400
(± 5300) 		33000
(± 2700)
  1. Quantification of compounds was performed in triplicate (n = 3)
  2. -: below limit of detection under the conditions tested
  3. * Method 2: Analysis of underivatized molecules by LC-MS/MS, this method was used to quantify compounds in the upper list section
  4. * Method 3: Analysis of methyl esters of FFA (FAMES) by GC-MS
  5. ** Tr. acid: Traumatic acid
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Plant Methods

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