Table 2 Quantification of Picolinyl ester derivatives of N. attenuata leaf extracts and comparison with two additional analytical methods.

0 min

30 min

60 min

wound

FAC

wound

FAC

Substance

RT

[M+H] ⁺

Picol. esters

Method 2 or 3*

Picol. esters

Method 2 or 3*

Picol. esters

Method 2 or 3*

Picol. esters

Method 2 or 3*

Picol. esters

Method 2 or 3*

ng gFW ^-1 (± SE)

ng gFW ^-1 (± SE)

ng gFW ^-1 (± SE)

ng gFW ^-1 (± SE)

ng gFW ^-1 (± SE)

ng gFW ^-1 (± SE)

ng gFW ^-1 (± SE)

ng gFW ^-1 (± SE)

ng gFW ^-1 (± SE)

ng gFW ^-1 (± SE)

JA-Ile

6.86

415

-

1.1

(± 0.14)

39

(± 4.0)

46

(± 4.8)

92

(± 6.0)

81

(± 2.0)

83

(± 9.0)

97

(± 4.0)

158

(± 16)

163

(± 19)

SA

8.77

230

193

(± 31)

173

(± 23)

203

(± 24)

212

(± 19)

182

(± 15)

196

(± 11)

216

(± 22)

189

(± 17)

116

(± 18)

129

(± 22)

ABA

9.01

356

257

(± 7)

225

(± 8.0)

192

(± 35)

246

(± 9.0)

274

(± 16)

232

(± 14)

264

(± 6.0)

242

(± 10)

277

(± 12)

270

(± 14)

ICA

9.08

253

-

-

-

-

-

-

-

-

-

-

CA

9.34

240

-

-

-

-

-

-

-

-

-

-

JA

9.43

302

137

(± 20)

152

(± 23)

937

(± 150)

845

(± 95)

1845

(± 125)

1586

(± 110)

1564

(± 180)

1326

(± 42)

3820

(± 350)

3149

(± 223)

Traumatol

9.85

306

-

-

-

-

-

-

-

-

-

-

dnOPDA

9.85

356

-

-

-

-

-

-

-

-

-

-

IAA

9.94

267

-

-

-

-

-

-

-

-

-

-

Traumatin

9.96

304

11.3

(± 5.6)

14.4

(± 7.8)

17.1

(± 4.8)

15.2

(± 2.3)

14.6

(± 3.4)

12.4

(± 2.7)

13.8

(± 1.9)

15

(± 5.2)

10.7

(± 3.6)

12.2

(± 1.6)

Tr. acid**

10.15

320

-

5

(± 0.96)

19.6

(± 3.2)

24.3

(± 6.7)

23.8

(± 6.1)

20.4

(± 4.8)

17.1

(± 2.5)

19.6

(± 2.3)

20.9

(± 1.9)

21.4

(± 4.2)

OPC4:0

10.46

330

-

-

-

-

12.6

(± 8.1)

-

10.3

(± 4.9)

-

18.5

(± 4.7)

-

OPC6:0

10.67

358

-

-

-

-

5.3

(± 2.9)

-

8.6

(± 3.5)

-

11.2

(± 5.6)

-

OPDA

10.89

384

107

(± 60)

91

(± 53)

323

(± 47)

386

(± 45)

291

(± 11)

322

(± 15)

180

(± 45)

184

(± 15)

301

(± 31)

313

(± 3.0)

GA₃

11.27

438

-

-

-

-

-

-

-

-

-

-

OPC8:0

11.41

386

-

-

-

-

-

-

-

-

-

-

GA₄

11.99

424

-

-

-

-

-

-

-

-

-

-

18:3

12.83

370

446

(± 38)

398

(± 24)

480

(± 53)

497

(± 37)

523

(± 30)

497

(± 42)

605

(± 20)

596

(± 31)

476

(± 33)

424

(± 31)

16:1

13.2

346

52

(± 9)

26

(± 23)

41

(± 3.0)

22

(± 20)

48

(± 12)

51

(± 32)

91

(± 26)

147

(± 96)

103

(± 13)

121

(± 83)

16:2

10.93

344

-

-

-

-

-

-

-

-

-

-

16:3

10.29

342

45.6

(± 12)

-

41.1

(± 2.0)

28.2

(± 23.0)

64.1

(± 19)

40.5

(± 21)

130.2

(± 10)

80.9

(± 39)

37.2

(± 3.0)

-

18:2

14.22

372

245

(± 16)

190

(± 17)

251

(± 8.0)

189

(± 13)

316

(± 45)

398

(± 23)

377

(± 43)

415

(± 26)

333

(± 209)

367

(± 25)

16:0

14.53

348

21100

(± 3000)

22300

(± 4150)

16600

(± 930)

14100

(± 2300)

18600

(± 2800)

15400

(± 4700)

23200

(± 3200)

21600

(± 3000)

14600

(± 900)

15600

(± 3300)

18:1

14.86

374

625

(± 47)

502

(± 66)

603

(± 22)

572

(± 72)

805

(± 59)

716

(± 28)

791

(± 84)

778

(± 47)

894

(± 36)

813

(± 51)

18:0

17.59

376

46800

(± 12900)

44600

(± 7300)

47900

(± 1200)

33400

(± 9300)

49000

(± 5100)

36700

(± 4600)

45700

(± 6200)

41300

(± 3300)

39400

(± 5300)

33000

(± 2700)