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Figure 2 | Plant Methods

Figure 2

From: A rapid and sensitive method for the simultaneous analysis of aliphatic and polar molecules containing free carboxyl groups in plant extracts by LC-MS/MS

Figure 2

Reaction mechanisms for the formation of Picolinyl ester derivatives of carboxylic acids. The carboxyl group is first activated by reaction with 1,1'-carbidiimidazole to form the active amid 1. 1 is then transesterified with 3-(hydroxymethyl)-pyridine to form the Picolinyl ester derivative 2. After collision induced dissociation (CID), the major ion fragments obtained are m/z = 92 and m/z = 108.

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Plant Methods

ISSN: 1746-4811