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Figure 2 | Plant Methods

Figure 2

From: A simple, flexible and efficient PCR-fusion/Gateway cloning procedure for gene fusion, site-directed mutagenesis, short sequence insertion and domain deletions and swaps

Figure 2

DNA 'domain deletion' protocol. Schematic representation of a 'domain deletion' using PCR amplification of the two terminal regions of the entry clone adjacent to the DNA domain to be deleted (designated by 'X'). F and R - represent M13 universal primers. SF and SR are complementary PCR primers consisting of two fused sections (a and b) that match regions flanking the deleted domain. The overlap extension results in assembly of the two terminal regions with overlapping ends. The new fragment with deleted DNA domain is directly LR clonase cloned into a binary destination vector producing binary expression vector, (for details see Fig. 1).

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Plant Methods

ISSN: 1746-4811