Implementation of two high through-put techniques in a novel application: detecting point mutations in large EMS mutated plant populations

Table 3 Target genes and associated primer sequences.

Gene	Product size	Primer sequences
Region		Forward primers (5' - 3')	Reverse Primers (5' - 3')
*Auxin Response Factor 7*
ARF7_1	307 bp	AATTCAGAGTTATGGCACGCTTG	TCGAGAACTCCCAGCCAATATGTG
ARF7_2	418 bp	CCTCCTCCCATAAGTTGTATGAAAC	ACAGTGTTACCCCATTAGTAGTTCC
ARF7_3	469 bp	TCCTTGCTGCTGCTGCTCATGC	GCAGGAAGGGCTGTACTATGACCAC
*Expansin 1*
Exp_1	307 bp	TACAGCCAAGGATACGGAGTT	ATGGGATCCTGCGATAAGTT
*Phytoene Synthase*
PSY-1	220 bp	CATGGAATCAGTCCGGGAGGGA	CTTCACCAAGGCTGCCTGCC
PSY-2	366 bp	TCCTCCCTTTTTCTCCACTTCAAGC	AAGCCCTCAGCAAAAGTGACATCA
PSY-3	368 bp	TTCAATAGCGTAATTGTCTAACCTTCCA	ACCGGATAACCGAAGAGCTCA
*Proline Dehydrogenase 2*
ProDH_1	403 bp	GTGGAACATGCCACCGATAATGAATC	TCAATTGCAGGTTGAATGGTTGTG
ProDH_2	301 bp	AAAGATGATCAGCCTTTGATATTCGG	CCTGATTCAATGTTATGAGTGGCGAG
ProDH_3	234 bp	AAAACTTGCTGCAACCAAAGCTATAG	TGTCGAATGCCGATGTAGACAGCATG
*Sucrose Synthase 2*
Sus2-1	463 bp	TTTTCTGTGGAGTGAGGATTGTAGCTAA	GATAAGAGGAAAAACACCACAGACCTGC
Sus2-2	449 bp	TCGTGTGAAGAATTTAACCGGACTTGT	TTGCATTTCAAAGAAATCAGCTAGCA

ARF7: Auxin Response factor 7, GenBank: EF121545.1; Exp_1: Expansin 1, GenBank: U82123.1; PSY: Phytoene Synthase GenBank: EF157834.1; ProDH: Proline Dehydrogenase, TC209088: http://compbio.dfci.harvard.edu/tgi/cgi-bin/tgi/tc_report.pl?gudb=Tomato&tc=TC209088; Sus2; Sucrose Synthase 2, GenBank: AJ011535.1

ISSN: 1746-4811