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Table 1 Adapter and primer details

From: High-throughput retrotransposon-based fluorescent markers: improved information content and allele discrimination

Primer

Sequence

ds Taq adapter

5'- ATGAGTCCTGAA-3'

3' – TACTCAGGACTTGC-5'

PPT

5'- [6FAM or HEX or neither]

ATTCACCAGCTTGAGGGGAG-3'

Taq adapter +2

[16 combinations]

ATGAGTCCTGAACGANNa

  1. a NN two bases of selection
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Plant Methods

ISSN: 1746-4811